Journal: Polymers

Article Title: Bacterial Inhibition and Osteogenic Potentials of Sr/Zn Co-Doped Nano-Hydroxyapatite-PLGA Composite Scaffold for Bone Tissue Engineering Applications

doi: 10.3390/polym15061370

Figure Lengend Snippet: MTT assay for osteoblasts cultured for 2 and 7 days on different scaffolds: (nHAp, nHAp-PLGA, 1% Zn/Sr-nHAp-PLGA, 2.5% Zn/Sr-nHAp-PLGA, and 4% Zn/Sr-nHAp-PLGA). *** p ≤ 0.001 compared to nHAp.

Article Snippet: In this study, primary rat osteoblasts’ cell line (ROb) was purchased from (Cell Applications Inc., San Diego, CA, USA) and all the tissue culture reagents were purchased from Sigma-Aldrich.

Techniques: MTT Assay, Cell Culture

Journal: Scientific Reports

Article Title: HIF1A overexpression promotes osteoblast differentiation through activation of autophagy to alleviate osteoporosis

doi: 10.1038/s41598-025-16046-w

Figure Lengend Snippet: HIF-1a overexpression promotes osteoblast formation and autophagy. A: Transmission electron microscopy to observe the autophagy level of osteoblasts in each group. B: The relative protein expression of osteoblast markers (OCN and OPG) and autophagy-associated indicators (BNIP3, Beclin1, ATG, LC3 II/I) were detected in the cells by western-blot assay (vs. GAPDH). n = 3. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: Rat osteoblasts (CP-R091, Procell, Wuhan, China) were first cultured in basal medium (α-MEM) containing 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 μg/ml streptomycin.

Techniques: Over Expression, Transmission Assay, Electron Microscopy, Expressing, Western Blot

Journal: Scientific Reports

Article Title: HIF1A overexpression promotes osteoblast differentiation through activation of autophagy to alleviate osteoporosis

doi: 10.1038/s41598-025-16046-w

Figure Lengend Snippet: HIF-1a overexpression promotes osteoblast formation and autophagy in rats. A: Expression of autophagy markers LC3II was detected in femoral tissues by immunohistochemistry (IHC). B: The relative protein expression of osteoblast markers (OCN and OPG) and autophagy-associated indicators (BNIP3, Beclin1, ATG, LC3 II/I) detected by western-blot assay (vs. GAPDH). C: Expression of BALP, PINP, TRACP-5B and CTX-I in serum was detected by ELISA. n = 6. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: Rat osteoblasts (CP-R091, Procell, Wuhan, China) were first cultured in basal medium (α-MEM) containing 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 μg/ml streptomycin.

Techniques: Over Expression, Expressing, Immunohistochemistry, Western Blot, Enzyme-linked Immunosorbent Assay